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This system involved the administration of anabolic steroids on rats, either orally or by injection (depending on the anabolic steroid being assessed)[3,4]. The authors reported that the mean body mass of the steroids subjects was significantly lower in rats that were administered steroids by injection than in the steroid‐administered rats, with the average difference being 2.9±1.6% of the total body weight or, in absolute terms, 20±8 times what would be expected if the rats that received the high doses of steroids was all a few grams of fat or lean tissue (5). The study by Weyer et al  assessed the metabolic outcome of steroid administration in rats on the basis of the body mass. They found that the amount of fat mass and muscle mass, as well as the percentage of lean tissue in the rats that were administered steroids during fasting and during the next 24 h, was significantly lower in rats that were given steroids by injection than in the steroid‐administered rats. These data were further confirmed by Czeisler et al , who reported that there were no significant differences in the metabolic risk factors measured by the FFM in lean vs. fat rats that were administered steroids by injection or by gavage. It should be noted that both studies used a high dose (10 mg/kg injection) or a low dose (0.1 mg/kg injection), but did not specify the dose. The authors suggested that these findings may be due to the fact that the fat depot is somewhat larger in the steroid‐administered body, and the rats are given steroids for an extended period and there is a greater amount of time for the circulating steroid hormones to circulate in the rats than in the lean animals, thereby causing greater differences in circulating testosterone levels . In a follow‐up study by D'Amico et al , which took place during the same period as the previous study by Weyer et al, we evaluated the metabolic outcome in rats that were injected with either saline or 0.5 mg/kg testosterone enanthate. In the study by D'Amico et al , the animals were killed by decapitation and adipocytes were isolated. The fat was isolated from the fat pad of each fat organ using a needle and perfusion was performed using an Isorax kit. The percentage of visceral fat in the adipocytes or visceral fat in the organs was determined (3,4). They found that testosterone increased visceral fat in rats that were administered saline or testosterone enanthate, but not in those that were administered no steroids. The data from their study indicate that the effect of testosterone Similar articles: